COMPLEX PRODUCTION
Most proteins function as part of large dynamic complexes organized in response to external or intracellular signal. At NMX, we support our clients by producing these biologically relevant complexes in-vitro, enabling detailed study of their structure, interactions, and roles in cellular function.
These complexes are composed of protein-protein or protein-nucleic acid interactions, but can also involve other components (i.e. small molecules) to mimic a specific native-like cellular context. Identification and validation of specific binding partners is key to understanding the role of complexes in signaling pathways, localization, and overall biological function.
Why NMX?
From a therapeutic point of view, modulating specific biomolecule interactions relies on good understanding of complex formation. At NMX, we bring a deep expertise in the design, expression, and reconstitution of molecular complexes to provide realistic working units of biological complexes.
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We support your project from component expression and purification to controlled complex assembly and in-depth validation. Whether you're studying a stable protein dimer or a transient protein-RNA interaction, we help recreate the biological context needed for confident analysis and applications ranging from structural biology to drug discovery.
Complex Production
We routinely reconstitute and characterize:
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Protein-protein complexes
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Protein-nucleic acid assemblies
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Multicomponent assemblies
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Our Expertise Includes:
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In-house expression and purification of each complex component
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Controlled, stepwise, or co-expression-based assembly
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Use of isotope labeled proteins for NMR and biophysical assays
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Custom purification workflows using affinity, ion exchange (IEX), and size exclusion (SEC) chromatography
